The incorporation of 14C-leucine in a cell free system of intestine mucosa is diminished in aging. 2. The absence of 2-mercaptoethanol in the system of incorporation increases the age differences. 3. By combination of a system from intestine with liver preparation we observed a participation of both the microsomes and the postmicrosomal supernatant. 4. The activity of Ribonuclease is not changes in the aging process. Our results demonstrated the alterations of the intermitotic cells in aging.