单一患者体内肠球菌之间基因转移的可能性以及混淆分型结果的可能性。
Potential for gene transfer among enterococci from a single patient and the possibility of confounding typing results.
作者信息
Petts D N, Noble W C, Howell S A
机构信息
Department of Microbiology, Basildon Hospital, Nethermayne, United Kingdom.
出版信息
J Clin Microbiol. 1997 Jul;35(7):1722-7. doi: 10.1128/jcm.35.7.1722-1727.1997.
Abstract
The results of typing methods used in a study of the epidemiology of enterococci in burn patients showed inconsistencies. The possibility that these inconsistencies were the result of gene acquisition or loss was investigated by using 12 isolates of Enterococcus faecalis from a single patient. In vivo and in vitro exchange and loss of genes were observed. The study showed that the typing results for isolates from this patient can be modified by known and demonstrated genetic elements; as a result, the isolates could be divided into between three and seven strains. In the present study, the SmaI digestion patterns gave the most consistent results, correctly identifying the transconjugants as indistinguishable from recipient strain 196R.
摘要
一项关于烧伤患者肠球菌流行病学研究中所使用的分型方法结果显示存在不一致性。通过使用来自一名患者的12株粪肠球菌分离株，对这些不一致性是基因获得或缺失的结果这一可能性进行了调查。观察到了体内和体外基因的交换与缺失。该研究表明，来自该患者的分离株的分型结果可被已知且已证实的遗传元件所改变；因此，这些分离株可被分为3至7个菌株。在本研究中，SmaI酶切图谱给出了最一致的结果，正确地将转接合子鉴定为与受体菌株196R无法区分。
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