Takeuchi F, Nabeta H, Kuwata S, Tanimoto K, Ito K
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Clin Exp Rheumatol. 1997 Mar-Apr;15(2):189-92.
The contribution of polymorphism of DMA and DMB alleles to the pathogenesis of Japanese RA was studied. The association of DM alleles with HLA-DRB1*0405 and *0802, which were positively and negatively susceptible to Japanese RA, respectively, is also discussed.
DMA and DMB typing was carried out in 91 Japanese RA patients and in 77 normal subjects by the PCR-RFLP (restriction fragment length polymorphism) method. HLA-DRB1*04 and *08 genotyping were carried out by the PCR-SSCP (single-stranded DNA conformation polymorphism) method.
Allele frequencies of DMB0101 and DMB0102 were slightly higher (52.2% and 27.0%) and the allele frequency of DMB0103 was slightly lower (25.8%) in RA, but these differences were not significant. The increase of DMB0102 was due to a negative association with HLA-DRB10802 [p < 0.05, pc = not significant (NS)]. The decrease of DMB0103 was due to a positive association with DRB10802 (p < 0.005, pc < 0.05). The increase of DMB0101 was possibly due to a weak association with HLA-DRB1*0405, (p = NS). Positivity of rheumatoid factor did not affect the prevalence of DMA and DMB alleles.
Association analysis among DMA, DMB and DRB1 (0405 and 0802) indicate that slight increases or decreases in DMB0101, DMB0102 and DMB0103 are not primary indicators but reflect an increase in HLA-DRB1 0405 and a decrease in HLA-DRB1*0802 in Japanese RA.
研究DMA和DMB等位基因多态性对日本类风湿关节炎（RA）发病机制的影响。还讨论了DM等位基因与HLA - DRB10405和0802的关联，这两个基因分别对日本RA呈正易感性和负易感性。
采用PCR - RFLP（限制性片段长度多态性）方法对91例日本RA患者和77例正常受试者进行DMA和DMB分型。采用PCR - SSCP（单链DNA构象多态性）方法进行HLA - DRB104和08基因分型。
在RA患者中，DMB0101和DMB0102的等位基因频率略高（分别为52.2%和27.0%），而DMB0103的等位基因频率略低（25.8%），但这些差异无统计学意义。DMB0102的增加是由于与HLA - DRB10802呈负相关[p < 0.05，校正P值=无显著性差异（NS）]。DMB0103的减少是由于与DRB10802呈正相关（p < 0.005，校正P值< 0.05）。DMB0101的增加可能是由于与HLA - DRB1*0405的弱关联（p = NS）。类风湿因子阳性不影响DMA和DMB等位基因的患病率。
DMA、DMB和DRB1（0405和0802）之间的关联分析表明，DMB0101、DMB0102和DMB0103的轻微增减不是主要指标，而是反映了日本RA患者中HLA - DRB10405的增加和HLA - DRB1*0802的减少。
