Luboń H, Paleyanda R K
J. Holland Laboratory, American Red Cross, Rockville, MD, USA.
Thromb Haemost. 1997 Jul;78(1):532-6.
Vitamin K-dependent proteins are currently purified from pooled human plasma or produced in mammalian cell culture systems by recombinant DNA technology. Transgenic animals may provide an additional expression system for the production of these therapeutic proteins. Mice have been used to test hybrid genes which direct the expression of recombinant factor IX and Protein C to the mammary gland. Transgenic livestock have also been created that secrete into milk fully active Protein C at 0.3 mg/mL, and factor IX at 0.2 mg/mL. Thus, it is feasible to develop genetically manipulated livestock for the industrial production of vitamin K-dependent proteins.
维生素K依赖蛋白目前是从人混合血浆中纯化得到，或者通过重组DNA技术在哺乳动物细胞培养系统中生产。转基因动物可能为这些治疗性蛋白的生产提供另一种表达系统。小鼠已被用于测试将重组因子IX和蛋白C的表达导向乳腺的杂交基因。还培育出了转基因家畜，它们能在乳汁中分泌浓度为0.3毫克/毫升的完全活性蛋白C以及浓度为0.2毫克/毫升的因子IX。因此，开发基因改造家畜用于工业化生产维生素K依赖蛋白是可行的。
