Mahnke P F, Schulze S
Zentralbl Allg Pathol. 1977;121(4-5):456-64.
The rate of growth of isolated murine renal glomeruli was studied in vitro. Separation by screening was followed by cultivation in Carrel flacks using Eagle's medium, and the number of utilizable tests was 104. It was possible to cultivate glomeruli for up to 27 days and to observe the growth of cells. These were epithelial capsule cells, endothelial capillary cells and fibroblasts. The existence of mesangial cells not be convincingly demonstrated experimentally. The problems associated with the cultivation of isolated renal glomeruli are discussed with reference to data reported in the literature. Possible uses of this particular model are pointed out in some detail.
对分离出的小鼠肾小球的生长速率进行了体外研究。通过筛选分离后，在使用伊格尔培养基的卡雷尔培养瓶中进行培养，可用的测试数量为104次。肾小球可以培养长达27天，并观察细胞生长。这些细胞是上皮包膜细胞、内皮毛细血管细胞和成纤维细胞。系膜细胞的存在尚未通过实验得到令人信服的证明。结合文献报道的数据，讨论了与分离的肾小球培养相关的问题。详细指出了这个特定模型的可能用途。
