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[葡萄球菌血浆凝固酶。III. 利用血栓弹力图研究细胞外葡萄球菌凝固酶的特性]

[Staphylococcal plasmacoagulase. III. Use of thrombelastography to study the properties of extracellular staphylocoagulase].

作者信息

Grishina N B, Shraer D P

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1977 Oct(10):46-51.

PMID:919922
Abstract

A method of thromboelastography was applied to the study of the influence of supernatents of the S. aureus and S. epidermidis culture fluid on the process of blood coagulation. S. aureus No. 209 P, No. 8325-1 strains and mutants of the No. 209 P strain, resistant to proflavin induced a distinct shortening of the reaction time. In using S. epidermidis (strains D5/240 and D-5/48) and also in control experiments (plasma and medium) no shortening of the reaction period was observed. The data obtained permitted to recommend the use of thromboelastographic method for the purpose of detection of coagulase-positive staphylococcus strains and for the assessment of relative staphylocoagulase activity.

摘要

采用血栓弹力图方法研究金黄色葡萄球菌和表皮葡萄球菌培养液上清液对血液凝固过程的影响。209P号金黄色葡萄球菌、8325 - 1号菌株以及对原黄素耐药的209P号菌株突变体可使反应时间明显缩短。使用表皮葡萄球菌(D5/240和D - 5/48菌株)以及对照实验(血浆和培养基)时,未观察到反应期缩短。所获数据表明,血栓弹力图方法可用于检测凝固酶阳性葡萄球菌菌株以及评估相对葡萄球菌凝固酶活性。

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