Raus J, Love D N
Department of Veterinary Pathology, University of Sydney, New South Wales, Australia.
J Clin Microbiol. 1990 Feb;28(2):207-10. doi: 10.1128/jcm.28.2.207-210.1990.
The staphylocoagulases of Staphylococcus intermedius (39 strains from clinical samples from dogs and 1 strain from a pigeon) and Staphylococcus aureus (40 strains from nine different animal species) were compared by using the chromogenic methods of Engels et al. (W. Engels, M. Kemps, and C. P. A. van Boven, J. Clin. Microbiol. 14:496-500, 1981). S. intermedius produced staphylocoagulase which resembled that of S. aureus in its rate and method of action on prothrombin, but S. intermedius produced it in lesser amounts. Therefore, chromogenic substrate tests such as Chromozym-TH (Boehringer GmbH, Mannheim, Federal Republic of Germany) (as described by Engels et al.) can be used for the detection of staphylocoagulase in both of these species. However, to detect accurately the presence of S. intermedius staphylocoagulase by this method, preconcentration of the extracellular proteins or an extension of the reaction time of the test would be required. The method described by Engels et al. was designed specifically for clinical laboratories that handle human samples. Under those circumstances the method could be regarded as specific, enabling an identification of S. aureus only. However, as both S. aureus and S. intermedius give positive results in this test, in veterinary diagnostic laboratories, heavy reliance must not be placed on this test for determination of organisms to the species level.
运用恩格斯等人（W. Engels、M. Kemps和C. P. A. van Boven，《临床微生物学杂志》14:496 - 500，1981年）的显色法，比较了中间葡萄球菌（39株来自犬类临床样本，1株来自鸽类）和金黄色葡萄球菌（40株来自9种不同动物物种）的葡萄球菌凝固酶。中间葡萄球菌产生的葡萄球菌凝固酶在对凝血酶的作用速率和方式上与金黄色葡萄球菌相似，但中间葡萄球菌产生的量较少。因此，诸如Chromozym - TH（德国曼海姆勃林格股份有限公司）（如恩格斯等人所述）之类的显色底物试验可用于检测这两个物种中的葡萄球菌凝固酶。然而，要通过该方法准确检测中间葡萄球菌凝固酶的存在，需要对细胞外蛋白质进行预浓缩或延长试验的反应时间。恩格斯等人描述的方法是专门为处理人类样本的临床实验室设计的。在那种情况下，该方法可被视为特异性的，仅能鉴定金黄色葡萄球菌。然而，由于金黄色葡萄球菌和中间葡萄球菌在该试验中均呈阳性结果，在兽医诊断实验室中，不应过度依赖该试验来将生物体鉴定到物种水平。
