一个昼夜节律增强子介导黑腹果蝇中依赖PER的mRNA循环。
A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster.
作者信息
Hao H, Allen D L, Hardin P E
机构信息
Department of Biology, Texas A & M University, College Station 77843-3258, USA.
出版信息
Mol Cell Biol. 1997 Jul;17(7):3687-93. doi: 10.1128/MCB.17.7.3687.
Abstract
Genes expressed under circadian-clock control are found in organisms ranging from prokaryotes to humans. In Drosophila melanogaster, the period (per) gene, which is required for clock function, is transcribed in a circadian manner. We have identified a circadian transcriptional enhancer within a 69-bp DNA fragment upstream of the per gene. This enhancer drives high-amplitude mRNA cycling under light-dark-cycling or constant-dark conditions, and this activity is per protein (PER) dependent. An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment.
摘要
在从原核生物到人类的各种生物体中，都能发现受生物钟控制而表达的基因。在黑腹果蝇中，生物钟功能所需的周期（per）基因以昼夜节律的方式进行转录。我们在per基因上游69个碱基对的DNA片段中鉴定出了一个昼夜节律转录增强子。该增强子在明暗循环或持续黑暗条件下驱动高振幅的mRNA循环，且这种活性依赖于周期蛋白（PER）。这个69个碱基对片段中的一个E-box序列是高水平表达所必需的，但不是节律性表达所必需的，这表明PER通过该片段中的其他序列介导昼夜节律转录。
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