人类Supt5h蛋白是一种假定的染色质结构调节剂，在有丝分裂过程中会发生可逆磷酸化。

Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis.

作者信息

Stachora A A, Schäfer R E, Pohlmeier M, Maier G, Ponstingl H

机构信息

Deutsches Krebsforschungszentrum, Division for Molecular Biology of Mitosis, Heidelberg, Germany.

出版信息

FEBS Lett. 1997 Jun 2;409(1):74-8. doi: 10.1016/s0014-5793(97)00486-9.

DOI:10.1016/s0014-5793(97)00486-9

PMID:9199507

Abstract

The Saccharomyces cerevisiae proteins Spt4p, Spt5p and Spt6p are involved in transcriptional repression by modulating the structure of chromatin. From HeLa cells we have purified a human homologue of Spt5p, Supt5hp, and show here that the protein is reversibly phosphorylated in mitosis. The cloned cDNA predicts a protein of 1087 residues with 31% identity to yeast Spt5p. It includes an acidic N-terminus, a putative nuclear localization signal and a C-terminal region containing two different repeated motifs. One of them, with the consensus sequence P-T/S-P-S-P-Q/A-S/G-Y, is similar to the C-terminal domain in the largest subunit of RNA polymerase II.

摘要

酿酒酵母蛋白Spt4p、Spt5p和Spt6p通过调节染色质结构参与转录抑制。我们从人宫颈癌细胞系（HeLa细胞）中纯化出了Spt5p的人类同源物Supt5hp，并在此表明该蛋白在有丝分裂过程中会发生可逆磷酸化。克隆得到的cDNA预测编码一个由1087个氨基酸残基组成的蛋白质，与酵母Spt5p有31%的同一性。它包括一个酸性的N端、一个假定的核定位信号以及一个包含两种不同重复基序的C端区域。其中一个基序的共有序列为P-T/S-P-S-P-Q/A-S/G-Y，与RNA聚合酶II最大亚基的C端结构域相似。