Takakura N, Yoshida H, Ogura Y, Kataoka H, Nishikawa S, Nishikawa S
Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Japan.
J Histochem Cytochem. 1997 Jun;45(6):883-93. doi: 10.1177/002215549704500613.
We investigated the cells that express platelet-derived growth factor receptor alpha (PDGFR alpha) during mouse embryogenesis. PDGFR alpha expression has been identified by in situ hybridization or immunohistochemistry using polyclonal antibodies on tissue sectins. Because no immunostaining study using whole-mount specimens has been published to date, we established a new monoclonal antibody (MAb), APA5, for this purpose. Our results differed in that APA5 stained only the paraxial mesoderm, whereas other investigators concluded that most if not all mesodermal cells expressed PDGFR alpha. Moreover, we did not find PDGFR alpha expression in embryonic erythrocytes, which have been previously suggested to express PDGFR alpha. On the basis of our present results, we wish to revise the proposed PDGFR alpha expression as follows. At the pregastrulation stage, PDGFR alpha is expressed only in primitive endoderm, particularly that in the ectoplacental cone. On gastrulation, it is expressed at high levels in the paraxial mesoderm. This expression continues after its differentiation into the somite. Along with the differentiation and migration of the sclerotome, PDGFR alpha + cells begin to become distributed throughout the embryonal mesenchyme. During organogenesis, particularly intense staining is detected in regions of epithelial and mesenchymal interaction, such as the tooth bud and bronchi. In addition to mesodermal derivatives, the developing lens, apical ectodermal ridge, glial precursor, cardiac valves, and choroid plexus express PDGFR alpha. Our results with whole-mount immunostaining show that PDGFR alpha is abundantly expressed and may play important roles during embryogenesis.
我们研究了小鼠胚胎发育过程中表达血小板衍生生长因子受体α（PDGFRα）的细胞。PDGFRα的表达已通过原位杂交或使用多克隆抗体对组织切片进行免疫组织化学鉴定。由于迄今为止尚未发表使用整装标本的免疫染色研究，我们为此建立了一种新的单克隆抗体（MAb）APA5。我们的结果有所不同，因为APA5仅对轴旁中胚层染色，而其他研究人员得出结论，大多数（如果不是全部）中胚层细胞表达PDGFRα。此外，我们在胚胎红细胞中未发现PDGFRα的表达，而之前有研究表明胚胎红细胞表达PDGFRα。基于我们目前的结果，我们希望对PDGFRα的表达情况进行如下修正。在原肠胚形成前期，PDGFRα仅在内胚层中表达，特别是在胚外胎盘锥中的内胚层。在原肠胚形成时，它在轴旁中胚层中高水平表达。这种表达在其分化为体节后仍持续。随着硬骨节的分化和迁移，PDGFRα +细胞开始分布于整个胚胎间充质中。在器官发生过程中，在上皮和间充质相互作用的区域，如牙胚和支气管中检测到特别强烈的染色。除了中胚层衍生物外，发育中的晶状体、顶端外胚层嵴、神经胶质前体、心脏瓣膜和脉络丛也表达PDGFRα。我们整装免疫染色的结果表明，PDGFRα大量表达，并且可能在胚胎发育过程中发挥重要作用。
