Owatari S, Zhang J, Gao M, Tanabe-Tochikura A, Wakamiya N, Tsuchie H, Kurimura T
Department of Viral Infections, Osaka University, Japan.
Acta Virol. 1997 Feb;41(1):21-6.
Monocytes/macrophages have been known to play an important role in the initiation and propagation of human immunodeficiency virus 1 (HIV-1) infection. To analyze the function of these cells during the clinical asymptomatic period of infection, we examined the effect of murine peritoneal macrophages and human peripheral blood macrophages on two cell lines latently infected with HIV-1, a promonocytic cell line, U1, and a T-cell line, ACH-2. Monokines of the murine peritoneal macrophages induced significant viral expression in U1, but not in ACH-2 cells. Experiments employing transient transfection of U937 and CEM cells with HIV long terminal repeat (LTR)-chloramphenicol acetyl transferase (CAT) plasmids indicated that the effect of these monokines was due to specific activation of the HIV LTR. In contrast, supernatants of human macrophages induced viral expression in both ACH-2 and U1 cells. These results suggest that several monokines are active in regulating the transition from the clinical asymptomatic period of HIV infection to progression to acquired immunodeficiency syndrome (AIDS).
单核细胞/巨噬细胞在人类免疫缺陷病毒1（HIV-1）感染的起始和传播过程中发挥着重要作用。为了分析这些细胞在感染临床无症状期的功能，我们检测了小鼠腹腔巨噬细胞和人外周血巨噬细胞对两种潜伏感染HIV-1的细胞系的影响，一种是前单核细胞系U1，另一种是T细胞系ACH-2。小鼠腹腔巨噬细胞的单核因子在U1细胞中诱导了显著的病毒表达，但在ACH-2细胞中未诱导。用HIV长末端重复序列（LTR）-氯霉素乙酰转移酶（CAT）质粒瞬时转染U937和CEM细胞的实验表明，这些单核因子的作用是由于HIV LTR的特异性激活。相比之下，人巨噬细胞的上清液在ACH-2和U1细胞中均诱导了病毒表达。这些结果表明，几种单核因子在调节从HIV感染的临床无症状期到发展为获得性免疫缺陷综合征（AIDS）的转变过程中具有活性。
