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小鼠肾小球旁器的超微结构细胞化学(作者译)

[Ultrastructural cytochemistry of the mouse juxtaglomerular apparatus (author's transl)].

作者信息

Cantin M, El-Khatib E, Yunge L

出版信息

Pathol Biol (Paris). 1979 May;27(5):261-8.

PMID:92002
Abstract

The ultrastructural cytochemistry of the mouse juxtaglomerular apparatus has been studied. The specific granules of the juxtaglomerular cells were found to be argentaphobic when ultrathin sections of araldite-embedded renal cortex were stained according to the periodic acid-thio-carbohydrazide-silver proteinate technique of Thiery. This technique revealed an abundant glycogen of type beta in the specific granules and in the cytosol of these cells. The rim of specific granules was positive when ultrathin sections of glutaraldehyde-fixed, glycol methacrylate-embedded kidneys were stained with phosphotungstic-hydrochloric acids at a low pH. A reaction was also shown by the cell coat, lysosomes, autophagolysosomes, residual bodies and part of the Golgi complex. These results indicate that the specific granules of the mouse juxtaglomerular apparatus contains glycoproteins that are glycosylated in the Golgi complex.

摘要

对小鼠肾小球旁器的超微结构细胞化学进行了研究。当根据蒂埃里的高碘酸 - 硫代 - carbohydrazide - 蛋白银技术对用环氧树脂包埋的肾皮质超薄切片进行染色时,发现肾小球旁细胞的特定颗粒对嗜银反应呈阴性。该技术显示这些细胞的特定颗粒和细胞质中存在丰富的β型糖原。当用低pH值的磷钨酸 - 盐酸对戊二醛固定、甲基丙烯酸乙二醇酯包埋的肾脏超薄切片进行染色时,特定颗粒的边缘呈阳性。细胞被膜、溶酶体、自噬溶酶体、残余体和部分高尔基体复合物也显示出反应。这些结果表明,小鼠肾小球旁器的特定颗粒含有在高尔基体复合物中进行糖基化的糖蛋白。

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