Determination of acyclovir in human plasma by high-performance liquid chromatography.

A selective and sensitive isocratic high-performance liquid chromatographic method for the analysis of acyclovir in human plasma was described. Acid deproteinisation was used as sample treatment. Mean analytical recoveries were higher than 94% at low and high concentrations. The quantification limit was 0.1 mg/l for a plasma volume of 500 microl and precision study exhibits coefficients of variation lower than 5%. The method is suitable for therapeutic monitoring of acyclovir concentrations in organ-transplant recipients.