采用荧光检测的高效液相色谱法测定人血浆中阿昔洛韦的简易方法。

Simple high-performance liquid chromatographic method for the determination of acyclovir in human plasma using fluorescence detection.

作者信息

Peh K K, Yuen K H

机构信息

School of Pharmaceutical Sciences, University of Science, Malaysia, Penang.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):241-4. doi: 10.1016/s0378-4347(97)00041-8.

DOI:10.1016/s0378-4347(97)00041-8

PMID:9200543

Abstract

A simple high-performance liquid chromatographic method using fluorescence detection was developed for the determination of acyclovir in human plasma. The method entailed direct injection of the plasma sample after deproteination. It is both specific and sensitive with a detection limit of 30 ng/ml at a signal-to-noise ratio of 3:1, and is thus suitable for use in pharmacokinetic studies of acyclovir. The method had a mean absolute recovery of 96%, while the within-day and between-day coefficients of variation and percentages error were all less than 8%. The calibration curve was linear over a concentration range of 62.5-4000 ng/ml.

摘要

建立了一种采用荧光检测的简单高效液相色谱法测定人血浆中的阿昔洛韦。该方法需要在去蛋白后直接进样血浆样品。该方法特异性强、灵敏度高，在信噪比为3:1时检测限为30 ng/ml，因此适用于阿昔洛韦的药代动力学研究。该方法的平均绝对回收率为96%，日内和日间变异系数及误差百分比均小于8%。校准曲线在62.5 - 4000 ng/ml的浓度范围内呈线性。