Apostolidis A P, Triantaphyllidis C, Kouvatsi A, Economidis P S
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki, Macedonia, Greece.
Mol Ecol. 1997 Jun;6(6):531-42. doi: 10.1046/j.1365-294x.1997.d01-176.x.
To investigate the phylogenetic relationships and geographical structure among brown trout S. trutta L. Populations from the South Adriatic-Ionian and Aegean sea basins, mitochondrial DNA sequence comparisons were used. A 310-base-pair (bp) segment of the control region (D-loop), and an additional 280-bp segment of the cytochrome beta gene were sequenced from representatives of 13 brown trout populations. Phylogenetic analyses, conducted after combining the data presented with published data from other Eurasian brown trout, revealed four major phylogenetic groups, three of which were found widely distributed within the southern Balkan region. The phylogeographical patterns revealed by mtDNA represent one of the few cases where phylogenetic discontinuity in a gene tree exists without obvious geographical localization within a species' range and has most likely resulted from the differentiation of the major mtDNA clades during Messinian or early Pleistocene times. Finally, the genetic relationships among the populations suggested by mtDNA were generally not in accordance with either allozyme or morphological data.
为了研究亚得里亚海-爱奥尼亚海南部和爱琴海流域褐鳟（Salmo trutta L.）种群之间的系统发育关系和地理结构，研究人员使用了线粒体DNA序列比较方法。从13个褐鳟种群的代表中，对控制区（D-loop）的一段310个碱基对（bp）的片段以及细胞色素β基因的另外一段280 bp的片段进行了测序。在将所呈现的数据与来自其他欧亚褐鳟的已发表数据相结合后进行的系统发育分析，揭示了四个主要的系统发育组，其中三个在巴尔干半岛南部地区广泛分布。线粒体DNA揭示的系统地理格局是少数几个基因树中存在系统发育间断但在物种分布范围内没有明显地理定位的案例之一，很可能是在墨西拿期或更新世早期主要线粒体DNA分支分化的结果。最后，线粒体DNA所表明的种群之间的遗传关系通常与等位酶或形态学数据不一致。
