Marić Sasa, Susnik Simona, Simonović Predrag, Snoj Ales
University of Belgrade, Faculty of Biology, Institute of Zoology, Studentski trg 16, 11001 Belgrade, Serbia and Montenegro.
Genet Sel Evol. 2006 Jul-Aug;38(4):411-30. doi: 10.1186/1297-9686-38-4-411. Epub 2006 Jun 23.
In order to illuminate the phylogeography of brown trout (Salmo trutta) populations in the Balkan state of Serbia, the 561 bp 5'-end of mtDNA control region of 101 individuals originating from upland tributaries of the Danubian, Aegean and Adriatic drainages were sequenced and compared to corresponding brown trout sequences obtained in previous studies. Among 15 haplotypes found, 14 were considered native, representing the Danubian and Adriatic lineages of the brown trout, while one haplotype (ATcs1), found only in two individuals originating from two stocked rivers, corresponded to the Atlantic lineage and was considered introduced. Native haplotypes exhibited a strong geographic pattern of distribution: the Danubian haplotypes were strictly confined to the Danubian drainage, while the Adriatic haplotypes dominated in the Aegean and Adriatic drainages; most of the total molecular variance (69%) was attributed to differences among the drainages. Phylogenetic reconstruction, supplemented with seven haplotypes newly described in this study, suggested a sister position of the Atlantic-Danubian and Adriatic-Mediterranean-marmoratus ("southern") phylogenetic group, and pointed to the existence of a distinct clade, detected within the "southern" group. The data obtained confirmed our expectation of the existence of high genetic diversity in Balkan trout populations, and we recommend more widespread surveys covering trout stocks from the region.
为了阐明巴尔干半岛国家塞尔维亚褐鳟（Salmo trutta）种群的系统地理学，对来自多瑙河、爱琴海和亚得里亚海排水区域上游支流的101个个体的线粒体DNA控制区561 bp 5'端进行了测序，并与先前研究中获得的相应褐鳟序列进行了比较。在所发现的15个单倍型中，14个被认为是本地单倍型，代表褐鳟的多瑙河和亚得里亚海谱系，而仅在两条放养河流的两个个体中发现的一个单倍型（ATcs1）对应于大西洋谱系，被认为是引入的。本地单倍型呈现出强烈的地理分布模式：多瑙河单倍型严格局限于多瑙河排水区域，而亚得里亚海单倍型在爱琴海和亚得里亚海排水区域占主导地位；大部分总分子变异（69%）归因于排水区域之间的差异。系统发育重建，补充了本研究中新描述的七个单倍型，表明大西洋-多瑙河和亚得里亚海-地中海-大理石纹（“南方”）系统发育组的姐妹位置，并指出在“南方”组内存在一个独特的分支。所获得的数据证实了我们对巴尔干半岛鳟鱼种群存在高遗传多样性的预期，并且我们建议对该地区的鳟鱼种群进行更广泛的调查。
