Turbeville J M, Staton J L, Brown W M
Department of Biology, University of Michigan, Ann Arbor 48109-1048, USA.
Mol Mar Biol Biotechnol. 1997 Jun;6(2):141-3.
We report the first polymerase chain reaction (PCR) amplification of the complete mitochondrial genomes of a nemertean and a sipunculan worm in one piece using a recently published two-polymerase protocol for long and accurate DNA amplification. Successful amplification was achieved from nanogram quantities of both purified mitochondrial DNA (nemertean) and crude total DNA (sipunculan). This technique allows the rapid generation of sufficient quantities of entire mitochondrial DNAs for cloning and restriction fragment length polymorphism (RFLP) analyses, and thus will facilitate comparative studies of metazoan mitochondrial genomes.
我们报道了首次使用最近发表的一种用于长片段和精确DNA扩增的双聚合酶方案，一次性对一种纽形动物和一种星虫动物的完整线粒体基因组进行聚合酶链反应（PCR）扩增。从纳克量的纯化线粒体DNA（纽形动物）和粗制总DNA（星虫动物）中均成功实现了扩增。该技术能够快速产生足够数量的完整线粒体DNA用于克隆和限制性片段长度多态性（RFLP）分析，因此将有助于后生动物线粒体基因组的比较研究。
