Torres M I, Fernandez M I, Gil A, Rios A
Department of Cell Biology, Faculty of Sciences, University of Granada, Spain.
Dig Dis Sci. 1997 Jun;42(6):1322-8. doi: 10.1023/a:1018882913983.
The administration of thioacetamide in rats induces nodular cirrhosis of the liver, characterized by fibrous septae, parenchymal nodules, proliferation of the bile ducts, and excessive deposition of connective tissue elements. Nodular cirrhosis is also associated with changes in lipid metabolism, as shown by the accumulation of lipid droplets in the hepatocyte cytoplasm. Adequate nutritional support during cirrhosis is important to sustain liver function and promote recovery after the lesions have been induced. Supplementation with nucleotides may increase cellular proliferation and thus optimize hepatic recovery. The aim of this study was to investigate the effects of dietary nucleotide supplementation on the degree of fibrosis and steatosis in rats with liver cirrhosis induced by four months of oral intake of thioacetamide. The use of dietary nucleotides after thioacetamide administration was found to decrease the percentage area of fibrous septae. In animals with liver cirrhosis fed the nucleotide-supplemented diet for two weeks, the total area of fibrosis was reduced. Withdrawal of the hepatotoxic agent led to a decrease in the degree of steatosis in cirrhotic animals, which was significant in rats given the nucleotide-supplemented diet during a two-week recovery period. In conclusion, dietary nucleotides may be an important factor in the histological recovery of damaged liver in experimental cirrhosis.
给大鼠服用硫代乙酰胺可诱发肝脏结节性肝硬化，其特征为纤维间隔、实质结节、胆管增生以及结缔组织成分过度沉积。结节性肝硬化还与脂质代谢变化有关，肝细胞质中脂滴的积累就表明了这一点。肝硬化期间充足的营养支持对于维持肝功能以及促进损伤诱导后的恢复很重要。补充核苷酸可能会增加细胞增殖，从而优化肝脏恢复。本研究的目的是调查口服硫代乙酰胺四个月诱导肝硬化的大鼠补充膳食核苷酸对纤维化程度和脂肪变性的影响。结果发现，服用硫代乙酰胺后使用膳食核苷酸可降低纤维间隔的面积百分比。在喂食补充核苷酸饮食两周的肝硬化动物中，纤维化总面积减少。停用肝毒性药物导致肝硬化动物脂肪变性程度降低，在两周恢复期给予补充核苷酸饮食的大鼠中这一现象尤为明显。总之，膳食核苷酸可能是实验性肝硬化受损肝脏组织学恢复的一个重要因素。
