Fernández I, Torres I, Moreira E, Fontana L, Gil A, Rios A
Department of Cell Biology, University of Granada, Spain.
Dig Dis Sci. 1996 Jan;41(1):197-207. doi: 10.1007/BF02208605.
Patients with liver cirrhosis frequently show some degree of protein-energy malnutrition and obviously require nutritional support. In this study, the treatment of rats consisted of the ad libitum oral intake of a 300 mg/liter thioacetamide solution, used as drinking water for four months. Thioacetamide treatment produced a severe alteration in the plasma fatty acid profile with significant decreases of these, which mimicked changes described in human cirrhosis. This hepatotoxic agent causes nodular cirrhosis, with loss of the normal architecture of the liver and disruption of the vascular pattern. The goal of the study was to evaluate the influence of n-3 and n-6 series long-chain polyunsaturated fatty acid dietary supplementation in experimental animals and to assess the effects of those dietary components on structural recovery in the liver. Significant increases of saturated and monounsaturated fatty acids as well as n-6 polyunsaturated fatty acids were seen only in the animals given the n-6 polyunsaturated fatty acid supplemented diet. However, only rats given the standard diet exhibited some degree of histological regeneration.
肝硬化患者常表现出一定程度的蛋白质 - 能量营养不良，显然需要营养支持。在本研究中，大鼠的治疗方法是随意口服浓度为300毫克/升的硫代乙酰胺溶液，该溶液用作饮用水，持续四个月。硫代乙酰胺治疗导致血浆脂肪酸谱发生严重改变，这些脂肪酸显著减少，这模拟了人类肝硬化中描述的变化。这种肝毒性剂会导致结节性肝硬化，肝脏正常结构丧失，血管模式破坏。该研究的目的是评估n - 3和n - 6系列长链多不饱和脂肪酸膳食补充剂对实验动物的影响，并评估这些膳食成分对肝脏结构恢复的作用。仅在给予补充n - 6多不饱和脂肪酸饮食的动物中，饱和脂肪酸、单不饱和脂肪酸以及n - 6多不饱和脂肪酸显著增加。然而，只有给予标准饮食的大鼠表现出一定程度的组织学再生。
