肾细胞癌中N-乙酰葡糖胺基转移酶III、IV和V的变化
Changes in N-acetylglucosaminyltransferase III, IV and V in renal cell carcinoma.
作者信息
Zhu T Y, Chen H L, Gu J X, Zhang Y F, Zhang Y K, Zhang R A
机构信息
Key Laboratory of Glycoconjugate Research, Shanghai Medical University, China.
出版信息
J Cancer Res Clin Oncol. 1997;123(5):296-9. doi: 10.1007/BF01208642.
The activities of N-acetylglucosaminyltransferase (GnT) III, IV and V were determined in 10 cases of renal cell carcinoma (RCC) and compared with the normal kidney cortex (NKC) regions of the same kidney resected from RCC patients. It was found that the GnT III and GnT IV activities decreased consistently in all samples of RCC, while GnT V activity increased, decreased or did not change in different samples. The mean levels of GnT III and GnT IV activities in RCC were found to be very significantly lower than those of NKC on statistical analysis, but the mean value of GnT V activity was almost identical in RCC and NKC. The decrease in GnT activities in RCC were compatible with the decrease in bisecting N-acetylglucosamine (GlcNAc) and antennary number of complex-type N-glycans in gamma-glutamyltranspeptidase (gamma-GT) partially purified from RCCs as studied with concanavalin A (ConA) affinity column chromatography, which showed a decrease of unbound fraction and increase of bound fractions.
在10例肾细胞癌（RCC）病例中测定了N-乙酰葡糖胺基转移酶（GnT）III、IV和V的活性，并与从RCC患者切除的同一肾脏的正常肾皮质（NKC）区域进行了比较。结果发现，在所有RCC样本中，GnT III和GnT IV活性持续下降，而GnT V活性在不同样本中增加、下降或未发生变化。经统计学分析，RCC中GnT III和GnT IV活性的平均水平显著低于NKC，但RCC和NKC中GnT V活性的平均值几乎相同。用伴刀豆球蛋白A（ConA）亲和柱色谱法研究发现，RCC中GnT活性的降低与从RCC中部分纯化的γ-谷氨酰转肽酶（γ-GT）中平分型N-乙酰葡糖胺（GlcNAc）和复合型N-聚糖天线数量的减少相一致，这表现为未结合部分减少，结合部分增加。
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