Takai N, Kataoka M, Higuchi Y, Matsuura K, Yamamoto S
Department of Pathology, Oita Medical University, Japan.
J Leukoc Biol. 1997 Jun;61(6):736-44. doi: 10.1002/jlb.61.6.736.
Rat CD14 cDNA clones were isolated. The predicted protein sequence exhibits 82, 61.6, and 64% identity with the mouse, rabbit, and human CD14, respectively. The levels of rat CD14 mRNA expression in resident peritoneal macrophages (PM), alveolar macrophages (AM), and peripheral blood monocytes (BM) were constitutively high, whereas that in Kupffer cells (KC) was low. On intravenous injection with lipopolysaccharide (LPS), the expression of rat CD14 mRNA in KC increased markedly, whereas the increases in PM, AM, and BM were mild. Similar features of expression of rat CD14 in these cells were observed after stimulation with LPS in vitro. The level of tumor necrosis factor alpha (TNF-alpha) mRNA expression in KC after stimulation with LPS in vivo was comparable to that in PM, AM, and BM, whereas that of TNF-alpha mRNA expression in KC and PM after stimulation with LPS in vitro was lower than that in AM and BM. Interleukin (IL)-1 beta and iNOS mRNA expressions in KC after stimulation with LPS in vivo and in vitro were low, whereas those in PM, AM, and BM were high. Little or no expression of IL-6 was observed in KC after stimulation with LPS in vivo and in vitro, whereas higher expression was observed in PM, AM, and BM than in KC.
大鼠CD14 cDNA克隆被分离出来。预测的蛋白质序列与小鼠、兔子和人类的CD14分别具有82%、61.6%和64%的同源性。常驻腹膜巨噬细胞（PM）、肺泡巨噬细胞（AM）和外周血单核细胞（BM）中大鼠CD14 mRNA的表达水平持续较高，而枯否细胞（KC）中的表达水平较低。静脉注射脂多糖（LPS）后，KC中大鼠CD14 mRNA的表达显著增加，而PM、AM和BM中的增加较为轻微。在体外经LPS刺激后，在这些细胞中观察到大鼠CD14表达的类似特征。体内经LPS刺激后，KC中肿瘤坏死因子α（TNF-α）mRNA的表达水平与PM、AM和BM中的相当，而体外经LPS刺激后，KC和PM中TNF-α mRNA的表达低于AM和BM。体内和体外经LPS刺激后，KC中白细胞介素（IL）-1β和诱导型一氧化氮合酶（iNOS）mRNA的表达较低，而PM、AM和BM中的表达较高。体内和体外经LPS刺激后，KC中IL-6的表达很少或未观察到，而PM、AM和BM中的表达高于KC。
