Hopkins B A, Olson L D
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
Avian Dis. 1997 Apr-Jun;41(2):317-25.
The live avirulent PM-1 Pasteurella multocida vaccine, grown in brain-heart infusion broth, was evaluated and compared in two experiments with the Clemson University (CU) vaccine, which had been shown to be effective in preventing fowl cholera in turkeys. Experiment 1 was performed during warm environmental temperatures and Expt. 2 during cooler environmental temperatures. The PM-1 vaccine was comparable with the CU vaccine in protecting turkeys against challenge with virulent P. multocida but was considered no less virulent than the CU because turkeys died after vaccination with both the PM-1 and the CU vaccines. A significantly (P < 0.05) higher percentage of unvaccinated turkeys challenged during the cooler environmental temperatures died than did unvaccinated turkeys challenged during the warmer temperatures. A microtiter agglutination test demonstrated a significant (P < 0.01) correlation between the level of serum anti-P. multocida antibody found 1 wk after vaccination and survival after challenge with virulent P. multocida in Expt. 1 and a significant (P < 0.05) correlation between these parameters in Expt. 2. However, there was a significant (P < 0.01) negative correlation between serum anti-P. multocida antibody titer 1 wk after vaccination and body weight gained 4 wk after vaccination, but before challenge, in Expt. 1, suggesting that vaccination with the live vaccines may have had a negative effect on body weight gain. At 4 wk after challenge or 8 wk after vaccination in Expt. 2, there was also a highly significant (P < 0.001) negative correlation between these parameters in the surviving turkeys.
在脑心浸液肉汤中培养的减毒活PM - 1多杀性巴氏杆菌疫苗，在两项实验中与克莱姆森大学（CU）疫苗进行了评估和比较，该CU疫苗已被证明在预防火鸡禽霍乱方面有效。实验1在温暖的环境温度下进行，实验2在较凉爽的环境温度下进行。PM - 1疫苗在保护火鸡免受强毒多杀性巴氏杆菌攻击方面与CU疫苗相当，但由于接种PM - 1和CU疫苗后火鸡均有死亡，因此认为其毒性不低于CU疫苗。在较凉爽环境温度下接受攻击的未接种疫苗的火鸡死亡百分比显著（P < 0.05）高于在温暖温度下接受攻击的未接种疫苗的火鸡。微量滴定凝集试验表明，在实验1中，接种疫苗1周后血清抗多杀性巴氏杆菌抗体水平与用强毒多杀性巴氏杆菌攻击后的存活率之间存在显著（P < 0.01）相关性，在实验2中这些参数之间存在显著（P < 0.05）相关性。然而，在实验1中，接种疫苗1周后的血清抗多杀性巴氏杆菌抗体滴度与接种疫苗4周后但在攻击前体重增加之间存在显著（P < 0.01）负相关，这表明接种活疫苗可能对体重增加有负面影响。在实验2中，攻击后4周或接种疫苗后8周，存活火鸡的这些参数之间也存在高度显著（P < 0.001）负相关。
