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花粉粒消化率的显微分光光度法评估

Microspectrophotometric evaluation of digestibility of pollen grains.

作者信息

Franchi G G, Franchi G, Corti P, Pompella A

机构信息

Dept. Farmaco Chimico Tecnologico, University of Siena, Italy.

出版信息

Plant Foods Hum Nutr. 1997;50(2):115-26. doi: 10.1007/BF02436031.

DOI:10.1007/BF02436031
PMID:9201746
Abstract

Digestibility of pollen grains of poppy (Papaver rhoeas) and hazelnut (Corylus avellana) subjected to a human-like in vitro digestion with pancreatic enzymes was evaluated. Pollens showed different types of walls. Digestibility was determined for total protein and insoluble carbohydrate contents by means of a new application of microspectrophotometry. Results demonstrated that pollen grains of both species were only partly digested; after 24 h treatment, only 26% of carbohydrates and 48% of proteins were digested in poppy and only 3% and 59% in hazelnut. This is probably due to the difficulty of enzymes to penetrate the intine of pollen grains. The degree of digestion of insoluble carbohydrates varied in the studied species according to their chemical nature and their storage sites.

摘要

评估了罂粟(Papaver rhoeas)和榛子(Corylus avellana)的花粉粒在类似人体的体外胰酶消化条件下的消化率。花粉显示出不同类型的壁。通过显微分光光度法的新应用测定了总蛋白和不溶性碳水化合物含量的消化率。结果表明,两个物种的花粉粒仅被部分消化;处理24小时后,罂粟中只有26%的碳水化合物和48%的蛋白质被消化,榛子中分别只有3%和59%。这可能是由于酶难以穿透花粉粒的内壁。在所研究的物种中,不溶性碳水化合物的消化程度因其化学性质和储存部位而异。

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本文引用的文献

1
Anaphylactic reaction after ingestion of local bee pollen.食用本地蜂花粉后发生过敏反应。
Ann Allergy. 1981 Sep;47(3):154-6.
2
[Persorption of pollen and spores by intact intestinal mucosa].
Acta Allergol. 1974 Jun;29(3):165-75.
3
Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate.通过酶促诱导荧光评估花粉活力;荧光素二乙酸酯的细胞内水解
Stain Technol. 1970 May;45(3):115-20. doi: 10.3109/10520297009085351.
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Acute allergic reaction after composite pollen ingestion.复合花粉摄入后出现急性过敏反应。
J Allergy Clin Immunol. 1979 Oct;64(4):270-4. doi: 10.1016/0091-6749(79)90143-x.