Hamberg H, Brunk U, Ericsson J L, Jung B
Acta Pathol Microbiol Scand A. 1977 Sep;85(5):625-39.
Topoinhibited human glia cells in vitro were exposed to X-radiation generated by an 8-MeV linear accelerator at a dose of 20,000 rad. Transmission electron microscopy of irradiated cells at intervals varying between 30 min. and 5 days following irradiation revealed alterations mainly in the plasma membrane and the lysosomal vacuome. Increased ruffling of plasma membranes, augmented endocytosis and extensive intracellular autophagy developed within 24 hours after irradiation. The implications of the plasma membrane alterations are discussed, and a tentative model covering possible mechanism involved in the development of autophagic vacuoles is presented. The possibility is entertained that alternative mechanism may be operative during the formation of the autophagic vacuole in irradiated glia cells. The origin of the isolation membrane appears to be (a) preexisting lysosomes; and (b) flattened vacuolar cytoplasmic elements.
体外经拓扑异构酶抑制的人神经胶质细胞接受了由一台8兆电子伏特直线加速器产生的剂量为20000拉德的X射线辐射。在辐射后30分钟至5天的不同时间间隔对受辐照细胞进行透射电子显微镜检查，结果显示主要是质膜和溶酶体空泡系统发生了改变。辐射后24小时内，质膜褶皱增加、内吞作用增强以及广泛的细胞内自噬现象出现。讨论了质膜改变的影响，并提出了一个涵盖自噬泡形成可能机制的初步模型。人们认为在受辐照神经胶质细胞自噬泡形成过程中可能存在其他作用机制。隔离膜的来源似乎是：(a) 预先存在的溶酶体；以及 (b) 扁平的空泡状细胞质成分。
