聚（ADP - 核糖基）化是否调节DNA甲基化模式？

Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern?

作者信息

Zardo G, D'Erme M, Reale A, Strom R, Perilli M, Caiafa P

机构信息

Department of Biomedical Sciences and Technologies, University of L'Aquila, I-67100 L'Aquila, Italy.

出版信息

Biochemistry. 1997 Jul 1;36(26):7937-43. doi: 10.1021/bi970241s.

DOI:10.1021/bi970241s

PMID:9201939

Abstract

The existence of a possible correlation between poly(ADP-ribosyl)ation and DNA methylation processes was investigated. In vivo and in vitro experiments were carried out on L929 mouse fibroblasts preincubated for 24 h with or without 3-aminobenzamide, a well-known inhibitor of poly(ADP-ribose) polymerase. Both experimental approaches evidenced a close relationship between these two important nuclear enzymatic mechanisms, suggesting that the poly(ADP-ribosyl)ated isoform of H1 histone and/or long and branched protein-free ADP-ribose polymers could act as protecting agents against full methylation of the CpG dinucleotides in genomic DNA.

摘要

研究了聚（ADP-核糖基）化与DNA甲基化过程之间可能存在的相关性。对L929小鼠成纤维细胞进行体内和体外实验，这些细胞在有或没有3-氨基苯甲酰胺（一种众所周知的聚（ADP-核糖）聚合酶抑制剂）的情况下预孵育24小时。两种实验方法都证明了这两种重要的核酶机制之间的密切关系，表明H1组蛋白的聚（ADP-核糖基）化异构体和/或长链和分支的无蛋白ADP-核糖聚合物可以作为防止基因组DNA中CpG二核苷酸完全甲基化的保护剂。