Quere F, Deschamps A, Urdaci M C
Laboratoire de Microbiologie et Biotechnologie (ISTAB-ENITA), Université Bordeaux I, Talence, France.
J Appl Microbiol. 1997 Jun;82(6):783-90. doi: 10.1046/j.1365-2672.1997.00157.x.
A 300 bp DNA fragment of Lactobacillus plantarum isolated by randomly amplified polymorphic DNA (RAPD) analysis was cloned and sequenced. This fragment was tested using a dot-blot DNA hybridization to technique for its ability to identify Lact. plantarum strains. This probe hybridized with all Lact. plantarum strains tested and with some strains of Lact. pentosus, albeit more weakly. Two internal primers of this probe were selected (LbP11 and LbP12) and polymerase chain reaction (PCR) was carried out. All Lact. plantarum strains tested amplified a 250 bp fragment contrary to the other LAB species tested. This specific PCR for Lact. plantarum was also performed from colonies grown on MRS medium with similar results. These methods enabled the rapid and specific detection and identification of Lact. plantarum.
通过随机扩增多态性DNA（RAPD）分析分离出植物乳杆菌的一段300 bp DNA片段，并进行克隆和测序。使用斑点印迹DNA杂交技术测试该片段识别植物乳杆菌菌株的能力。该探针与所有测试的植物乳杆菌菌株以及一些戊糖乳杆菌菌株杂交，尽管杂交较弱。选择该探针的两个内部引物（LbP11和LbP12）并进行聚合酶链反应（PCR）。与其他测试的乳酸菌物种相反，所有测试的植物乳杆菌菌株都扩增出一个250 bp的片段。也从在MRS培养基上生长的菌落中进行了针对植物乳杆菌的特异性PCR，结果相似。这些方法能够快速、特异性地检测和鉴定植物乳杆菌。
