Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, 842 15 Bratislava, Slovakia.
Biomed Res Int. 2013;2013:760298. doi: 10.1155/2013/760298. Epub 2013 Sep 4.
One hundred and twenty-five acid-resistant presumptive lactobacilli were isolated from Slovak Bryndza cheese and screened for their antimicrobial activity against eight bacterial pathogens using spot agar assay. Out of twenty-six Lactobacillus strains with strong inhibition activity, twenty were identified as Lactobacillus plantarum and six as Lactobacillus fermentum. The most active eleven L. plantarum isolates were further characterized in vitro for some probiotic and safety properties. Only three isolates K10, K21, and ZS07 showed the ability to grow over 50% in the presence of 0.3% bile. Strong deconjugation efficiency was determined for CK06 and K21. The highest β -galactosidase activity was shown in isolates ZS11, B01, CK06, and ZS07. Only three of the strains had the ability to produce tyramine: CK06, LM1, and ZS11. Strains K09, K21, ZS11, and ZS15 were susceptible to all tested antibiotics. Analysis of the results confirmed the L. plantarum isolates ZS07 and K21 as the most suitable for probiotic use, due to their desirable probiotic and safety characteristics.
从斯洛伐克 Bryndza 奶酪中分离出 125 株耐酸假定乳杆菌，并通过点琼脂试验筛选其对 8 种细菌病原体的抗菌活性。在 26 株具有强抑制活性的乳杆菌菌株中，有 20 株鉴定为植物乳杆菌，6 株鉴定为发酵乳杆菌。最具活性的 11 株植物乳杆菌分离株进一步在体外对一些益生菌和安全性特性进行了表征。只有 3 株分离株 K10、K21 和 ZS07 表现出在 0.3%胆汁存在下生长超过 50%的能力。CK06 和 K21 表现出很强的脱共轭效率。ZS11、B01、CK06 和 ZS07 分离株的β-半乳糖苷酶活性最高。只有 3 株菌株有产生酪胺的能力：CK06、LM1 和 ZS11。菌株 K09、K21、ZS11 和 ZS15 对所有测试的抗生素均敏感。结果分析证实，由于其理想的益生菌和安全性特性，植物乳杆菌分离株 ZS07 和 K21 最适合用作益生菌。
