Landesman Y, Sokol S Y
Department of Microbiology and Molecular Genetics, Harvard Medical School, Beth Israel Hospital, Boston, MA 02215, USA.
Mech Dev. 1997 May;63(2):199-209. doi: 10.1016/s0925-4773(97)00041-5.
Xwnt-2b is a novel member of the Wnt gene family and is 73-74% similar to human and mouse Wnt-2 proteins. Starting from stage 15, Xwnt-2b transcripts are localized to a non-contiguous stripe in the anterior neural plate of the Xenopus embryo. In the tailbud, Xwnt-2b is expressed along the dorsoanterior side of the prosencephalon-mesencephalon boundary. At the tadpole stages, the brain-specific expression fades, but the total amount of Xwnt-2b mRNA does not decline due to activation of its expression in non-brain areas. Microinjection of Xwnt-2b mRNA into a ventral blastomere of 4-8-cell embryos results in the formation of complete secondary body axes. These results suggest that Xwnt-2b is a member of the axis-inducing Wnts and that it is involved in brain development and in later organogenesis.
Xwnt - 2b是Wnt基因家族的一个新成员，与人类和小鼠的Wnt - 2蛋白有73 - 74%的相似性。从第15阶段开始，Xwnt - 2b转录本定位于非洲爪蟾胚胎前神经板中的一条不连续条带。在尾芽中，Xwnt - 2b沿着前脑 - 中脑边界的背前侧表达。在蝌蚪阶段，脑特异性表达消失，但由于其在非脑区域的表达激活，Xwnt - 2b mRNA的总量并未下降。将Xwnt - 2b mRNA显微注射到4 - 8细胞胚胎的腹侧卵裂球中会导致完整的次生体轴形成。这些结果表明Xwnt - 2b是诱导轴形成的Wnts成员之一，并且它参与脑发育和后期器官发生。
