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Use of dot-immunobinding assay for visual detection of rinderpest antibodies in vaccinated cattle.

作者信息

Singh G, Singh I

机构信息

Department of Veterinary Public Health and Epidemiology, Punjab Agricultural University, Ludhiana, India.

出版信息

Trop Anim Health Prod. 1997 May;29(2):73-6. doi: 10.1007/BF02632321.

DOI:10.1007/BF02632321
PMID:9203306
Abstract

A dot-immunobinding (DIB) assay was used to detect rinderpest antibodies in cattle vaccinated with Kabete 'O' strain vaccine, using purified rinderpest virus. Of 120 serum samples from vaccinated and non-vaccinated animals, rinderpest antibodies were detected in 80%, 88.4% and 91.6% of samples at 2, 3 and 4 weeks postvaccination respectively. All the serum samples from non-vaccinated animals were negative. The DIB results had a good correlation with those of the micro neutralisation test. The technique is simple, easy to perform and suitable for routine use in detecting rinderpest antibodies following vaccination.

摘要

相似文献

1
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本文引用的文献

1
A dot-immunobinding assay for monoclonal and other antibodies.一种用于单克隆抗体及其他抗体的斑点免疫结合测定法。
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Microelisa test for detecting antibodies to rinderpest virus antigens.
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Simple and rapid dot-enzyme immunoassay for visual detection of rinderpest antibodies in bovine and caprine sera.用于肉眼检测牛和羊血清中牛瘟抗体的简单快速斑点酶免疫测定法。
Trop Anim Health Prod. 1986 Nov;18(4):209-16. doi: 10.1007/BF02359536.
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