Fickweiler S, Szeimies R M, Bäumler W, Steinbach P, Karrer S, Goetz A E, Abels C, Hofstädter F, Landthaler M
Department of Dermatology, University of Regensburg, Germany.
J Photochem Photobiol B. 1997 Apr;38(2-3):178-83. doi: 10.1016/s1011-1344(96)07453-2.
Indocyanine green (ICG; absorption peak in human plasma 805 nm) was investigated for ICG-mediated phototherapy in vitro. The cellular uptake of ICG (1 microM-50 microM) into HaCaT keratinocytes after an incubation period of 24 h increased up to an intracellular ICG concentration of 12.1 +/- 1.3 nmol per 10(6) cells. To examine dose dependent phototoxic effects in vitro, keratinocytes were incubated with 0 microM-50 microM ICG for 24 h and irradiated by a diode laser (805 nm) with different energy densities (0, 12, 24, 48 J cm-2). All applied ICG concentrations except for 5 microM yielded a cell killing effect in combination with irradiation depending significantly on ICG concentration and light dose. Cell viability for dark control and cells incubated with 50 microM ICG and irradiated with 48 J cm-2 was 0.82 +/- 0.15 and 0.07 +/- 0.02, respectively. Sodium azide (100 mM), a quencher of reactive oxygen species, inhibited significantly the cell killing using 50 microM ICG and 24 J cm-2. Taken together, photoactivation of ICG by irradiation with a diode laser was shown to induce effectively cell killing of HaCaT keratinocytes. Moreover, this effect was inhibited by sodium azide, thus irradiation of ICG might induce a photodynamic reaction.
研究了吲哚菁绿（ICG；在人体血浆中的吸收峰为805nm）用于体外ICG介导的光疗。在孵育24小时后，ICG（1μM - 50μM）进入HaCaT角质形成细胞的细胞摄取量增加，细胞内ICG浓度达到每10⁶个细胞12.1±1.3nmol。为了在体外检查剂量依赖性光毒性作用，将角质形成细胞与0μM - 50μM的ICG孵育24小时，并用不同能量密度（0、12、24、48J/cm²）的二极管激光（805nm）照射。除5μM外，所有应用的ICG浓度与照射相结合均产生细胞杀伤作用，这显著取决于ICG浓度和光剂量。暗对照以及用50μM ICG孵育并以48J/cm²照射的细胞的细胞活力分别为0.82±0.15和0.07±0.02。叠氮化钠（100mM），一种活性氧淬灭剂，显著抑制了使用50μM ICG和24J/cm²时的细胞杀伤作用。综上所述，用二极管激光照射激活ICG可有效诱导HaCaT角质形成细胞的细胞杀伤。此外这种作用被叠氮化钠抑制，因此ICG的照射可能诱导光动力反应。
