Geralde Mariana C, Leite Ilaiáli S, Inada Natalia M, Salina Ana Carolina G, Medeiros Alexandra I, Kuebler Wolfgang M, Kurachi Cristina, Bagnato Vanderlei S
University of São Paulo, São Carlos, Brazil
Federal University of São Carlos, São Carlos, Brazil.
Physiol Rep. 2017 Mar;5(5). doi: 10.14814/phy2.13190.
Infectious pneumonia is a major cause of morbidity/mortality, mainly because of the increasing rate of microorganisms resistant to antibiotics. Photodynamic Therapy (PDT) is emerging as a promising approach, as effects are based on oxidative stress, preventing microorganism resistance. In two previous studies, the in vitro inactivation of using indocyanine green (ICG) and infrared light source was a success killing 5 log colony-forming units (CFU/mL) with only 10 mol/L ICG. In this work, a proof-of-principle protocol was designed to treat lung infections by PDT using extracorporeal illumination with a 780 nm laser device and also ICG as photosensitizer. Hairless mice were infected with and PDT was performed two days after infection. For control groups, CFU recovery ranged between 10-10/mouse. For PDT group, however, no bacteria were recovered in 80% of the animals. Based on this result, animal survival was evaluated separately over 50 days. No deaths occurred in PDT group, whereas 60% of the control group died. Our results indicate that extracorporeal PDT has the potential for pneumonia treatment, and pulmonary decontamination with PDT may be used as a single therapy or as an antibiotics adjuvant.
感染性肺炎是发病/死亡的主要原因，主要是因为对抗生素耐药的微生物比例不断增加。光动力疗法（PDT）作为一种有前景的方法正在兴起，因为其作用基于氧化应激，可防止微生物产生耐药性。在之前的两项研究中，使用吲哚菁绿（ICG）和红外光源在体外使[此处原文缺失具体微生物名称]失活取得成功，仅用10 μmol/L的ICG就能杀死5个对数级的菌落形成单位（CFU/mL）。在这项工作中，设计了一个原理验证方案，通过使用780 nm激光设备进行体外照射以及ICG作为光敏剂，利用光动力疗法治疗肺部感染。将无毛小鼠感染[此处原文缺失具体微生物名称]，并在感染后两天进行光动力疗法。对于对照组，每只小鼠的CFU恢复范围在10⁵ - 10⁷之间。然而，对于光动力疗法组，80%的动物未检测到细菌。基于这一结果，分别在50天内评估动物存活率。光动力疗法组未出现死亡情况，而对照组有60%死亡。我们的结果表明，体外光动力疗法有治疗肺炎的潜力，并且光动力疗法进行肺部净化可作为单一疗法或抗生素辅助疗法。
