Marques M L, Silva J, Roseiro J C
Instituto Nacional de Engenharia e Tecnologia Industrial, IBQTA-Laboratório de Microbiologia Industrial, Azinhaga dos Lameiros à Estrada do Paço do Lumiar, Lisboa, Portugal.
Lett Appl Microbiol. 1997 Jun;24(6):435-7. doi: 10.1046/j.1472-765x.1997.00070.x.
Two organisms, CCMI507 and CCMI852, degrading undecylbenzenesulphonate (LAS) by the ortho- and meta-cleavage pathways were studied in cultures where glucose was used as carbon and energy source. CCMI507 (ortho-pathway) started the degradation of LAS at the beginning of the culture development in parallel with glucose utilization. The degradation followed a steady profile of degradation until 77% of LAS was degraded in the culture containing initially 5 mg l-1 of the compound and 81% in the cultures containing initially 10 and 20 mg l-1 of LAS, after 72 h fermentation. The organism CCMI852 (meta-pathway) started degrading the compound only after 20 h, when 75% of glucose was spent and well within the stationary-phase. After 72 h fermentation the level of degradation by CCMI852 varied from 70% (5 mg l-1 of LAS) to around 75% (10 and 20 mg l-1 of LAS).
研究了两种通过邻位和间位裂解途径降解直链烷基苯磺酸盐(LAS)的菌株CCMI507和CCMI852,培养时以葡萄糖作为碳源和能源。CCMI507(邻位途径)在培养初期与葡萄糖利用同时开始降解LAS。降解过程呈现稳定的降解曲线,在含有初始浓度为5 mg l-1该化合物的培养物中,72小时发酵后77%的LAS被降解;在含有初始浓度为10和20 mg l-1 LAS的培养物中,81%的LAS被降解。菌株CCMI852(间位途径)仅在20小时后开始降解该化合物,此时75%的葡萄糖已消耗殆尽且处于稳定期。72小时发酵后,CCMI852的降解水平从70%(5 mg l-1 LAS)到约75%(10和20 mg l-1 LAS)不等。