γ干扰素和转化生长因子-β在红细胞介导的巨噬细胞一氧化氮产生调节中的作用。
A role for interferon-gamma and transforming growth factor-beta in erythroid cell-mediated regulation of nitric oxide production in macrophages.
作者信息
Seledtsova G V, Seledtsov V I, Taraban V Y, Samarin D M, Kozlov V A
机构信息
Department of Experimental Immunology, Institute of Clinical Immunology, Novosibirsk, Russia.
出版信息
Immunology. 1997 May;91(1):109-13. doi: 10.1046/j.1365-2567.1997.00201.x.
Interferon-gamma (IFN-gamma) and transforming growth factor-beta (TGF-beta) are known to be a potent inducer and inhibitor for macrophage (Mo) activation process, respectively. In the present study we established that the nucleated erythroid cells (NEC) separated from the spleens of adult (CBA x C57BL/6)F1 (CBF, H-2k/H-2d) mice following phenylhydrazine treatment are potentially capable of inducing nitric oxide (NO) production in thioglycollate broth-elicited peritoneal macrophages (Mo). The stimulating effect of both NEC and their culture supernatant on NO secretion by Mo was most apparent in the presence of bacterial lipopolysaccharide (LPS) and neutralizing antibodies (Abs) to TGF-beta and was largely reversed by the addition to the culture of neutralizing Abs to IFN-gamma. Collectively these results suggest that NEC, through production of IFN-gamma and TGF-beta, may exert a regulatory influence on development and functionality of cells pertaining to monocyte (Mc)/Mo lineage.
已知γ干扰素（IFN-γ）和转化生长因子-β（TGF-β）分别是巨噬细胞（Mo）激活过程的强效诱导剂和抑制剂。在本研究中，我们证实，经苯肼处理后从成年（CBA×C57BL/6）F1（CBF，H-2k/H-2d）小鼠脾脏中分离出的有核红细胞（NEC）有潜力诱导巯基乙酸肉汤诱导的腹腔巨噬细胞（Mo）产生一氧化氮（NO）。在存在细菌脂多糖（LPS）以及针对TGF-β的中和抗体（Abs）时，NEC及其培养上清液对Mo分泌NO的刺激作用最为明显，而向培养物中添加针对IFN-γ的中和抗体后，这种作用在很大程度上被逆转。总体而言，这些结果表明，NEC可能通过产生IFN-γ和TGF-β，对与单核细胞（Mc）/Mo谱系相关的细胞的发育和功能发挥调节作用。
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