van Os R, de Witte T, Dillingh J H, Mauch P M, Down J D
Department of Radiobiology, University of Groningen, The Netherlands.
Leukemia. 1997 Jul;11(7):1045-8. doi: 10.1038/sj.leu.2400704.
The role of presensitizing murine recipients with donor spleen cells prior to T cell-depleted or -repleted H-2 compatible allogeneic bone marrow transplantation (BMT) was investigated at two different doses of total body irradiation (TBI). Recipients that were presensitized with 2 x 10(7) irradiated donor spleen cells at 1 week before a sublethal dose of 6 Gy TBI and BMT showed no evidence of donor blood chimerism while unsensitized recipients showed about 80% donor engraftment as determined by blood Gpi phenotyping. After raising the TBI dose to 9.5 Gy an increase in mortality from marrow failure was observed in presensitized animals. No significant engraftment-promoting effect of up to 2 x 10(6) T cells (20% of total marrow dose) was seen either in presensitized or unsensitized mice. It can be concluded that presensitized recipients are more susceptible to acute marrow rejection and that T cells added to the bone marrow did not influence the level of donor engraftment in these recipients.
在两种不同剂量的全身照射（TBI）条件下，研究了在T细胞去除或补充的H-2相容同种异体骨髓移植（BMT）之前，用供体脾细胞对小鼠受体进行预致敏的作用。在6 Gy亚致死剂量的TBI和BMT前1周，用2×10⁷个经照射的供体脾细胞进行预致敏的受体，未显示出供体血液嵌合体的迹象，而未致敏的受体通过血液Gpi表型分析显示约80%的供体植入。将TBI剂量提高到9.5 Gy后，在预致敏动物中观察到因骨髓衰竭导致的死亡率增加。在预致敏或未致敏的小鼠中，高达2×10⁶个T细胞（占总骨髓剂量的20%）均未显示出明显的促进植入作用。可以得出结论，预致敏的受体更容易发生急性骨髓排斥反应，并且添加到骨髓中的T细胞不会影响这些受体中供体植入的水平。
