在扫描透射电子显微镜下对聚合物化甲烷标记的噬菌体进行可视化观察。
Visualization of polymercurimethane-labeled for bacteriophage in the scanning transmission electron microscope.
作者信息
Lipka J J, Lippard S J, Wall J S
出版信息
Science. 1979 Dec 21;206(4425):1419-21. doi: 10.1126/science.92053.
PMID:92053
Abstract
Each of the 2700 coat proteins of fd bacteriophage was labeled with tetrakis(acetoxymercuri)methane (TAMM) or aquoglycylmethionineplatinum(II). The TAMM-labeled specimens reveal striking bright spots in the scanning transmission electron microscope which arise from clustering. Measurements of mass show increases consistent with the addition of four mercury atoms or one platinum atom, respectively, to each coat protein.
摘要
fd噬菌体的2700个衣壳蛋白中的每一个都用四（乙酰氧基汞）甲烷（TAMM）或水合甘氨酰甲硫氨酸铂（II）进行了标记。TAMM标记的样本在扫描透射电子显微镜下显示出由聚集产生的明显亮点。质量测量表明，每个衣壳蛋白分别增加了四个汞原子或一个铂原子，这与测量结果一致。
相似文献
1
Visualization of polymercurimethane-labeled for bacteriophage in the scanning transmission electron microscope.在扫描透射电子显微镜下对聚合物化甲烷标记的噬菌体进行可视化观察。
Science. 1979 Dec 21;206(4425):1419-21. doi: 10.1126/science.92053.
2
3
Bacteriophage PRD1 proteins: cross-linking and scanning transmission electron microscopy analysis.噬菌体PRD1蛋白：交联与扫描透射电子显微镜分析
Virology. 1993 Jun;194(2):570-5. doi: 10.1006/viro.1993.1296.
4
Tetrakis(acetoxymercuri)methane: a polymetallic reagent for labeling sulfur in nucleic acids.四（乙酰氧基汞）甲烷：一种用于核酸中硫标记的多金属试剂。
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1181-4. doi: 10.1073/pnas.75.3.1181.
5
Letter: Bacteriophage and bacteriocin as revealed by field emission source scanning electron microscope.信函：场发射源扫描电子显微镜下揭示的噬菌体和细菌素
J Electron Microsc (Tokyo). 1974;23(4):301-2.
6
Nuclear magnetic resonance of the filamentous bacteriophage fd.丝状噬菌体fd的核磁共振
Biophys J. 1980 Oct;32(1):531-48. doi: 10.1016/S0006-3495(80)84988-5.
7
Filamentous phage assembly: membrane insertion of the major coat protein.丝状噬菌体组装：主要衣壳蛋白的膜插入
Prog Clin Biol Res. 1981;64:389-400.
8
Flattening and shrinkage of bacteriophage T7 after preparation for electron microscopy by negative staining.通过负染色法制备用于电子显微镜观察的噬菌体T7后，其出现扁平化和收缩现象。
J Ultrastruct Res. 1977 Mar;58(3):235-43. doi: 10.1016/s0022-5320(77)90015-6.
9
High resolution scanning electron microscopy at the subcellular level.亚细胞水平的高分辨率扫描电子显微镜检查。
J Microsc. 1975 Mar;103(2):249-57. doi: 10.1111/j.1365-2818.1975.tb03900.x.
10
Electron microscope images of mercury atoms bound to DNA filament.
Ultramicroscopy. 1975 Jul;1(1):7-14. doi: 10.1016/s0304-3991(75)80004-0.
Zotero 插件