在扫描透射电子显微镜下对聚合物化甲烷标记的噬菌体进行可视化观察。
Visualization of polymercurimethane-labeled for bacteriophage in the scanning transmission electron microscope.
作者信息
Lipka J J, Lippard S J, Wall J S
出版信息
Science. 1979 Dec 21;206(4425):1419-21. doi: 10.1126/science.92053.
PMID:92053
Abstract
Each of the 2700 coat proteins of fd bacteriophage was labeled with tetrakis(acetoxymercuri)methane (TAMM) or aquoglycylmethionineplatinum(II). The TAMM-labeled specimens reveal striking bright spots in the scanning transmission electron microscope which arise from clustering. Measurements of mass show increases consistent with the addition of four mercury atoms or one platinum atom, respectively, to each coat protein.
摘要
fd噬菌体的2700个衣壳蛋白中的每一个都用四（乙酰氧基汞）甲烷（TAMM）或水合甘氨酰甲硫氨酸铂（II）进行了标记。TAMM标记的样本在扫描透射电子显微镜下显示出由聚集产生的明显亮点。质量测量表明，每个衣壳蛋白分别增加了四个汞原子或一个铂原子，这与测量结果一致。
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