四（乙酰氧基汞）甲烷：一种用于核酸中硫标记的多金属试剂。
Tetrakis(acetoxymercuri)methane: a polymetallic reagent for labeling sulfur in nucleic acids.
作者信息
Strothkamp K G, Lehmann J, Lippard S J
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1181-4. doi: 10.1073/pnas.75.3.1181.
Tetrakis(acetoxymercuri)methane binds to the sulfur atom of 6-thioguanosine and also to the 4-thiouridine residue of Escherichia coli tRNAVal. A 4:1 complex is formed between 6-thioguanosine and tetrakis(acetyoxymercuri)methane. Addition of 3 equivalents of N,N-dimethyl-2-amino-ethanethiol hydrochloride to tetrakis(acetoxymercuri)methane effectively blocks three of the four mercury atoms, rendering the compound monofunctional toward 6-thioguanosine. Under appropriate conditions, tetrakis(acetyoxymercuri)methane, in the presence or absence of N,N-dimethyl-2-amino-ethanethiol hydrochloride, binds to the 4-thiouridine residue in E. coli tRNAVal without forming intermolecular crosslinks. These results suggest that tetrakis(acetoxymercuri)methane will be a useful polymetallic reagent for labeling sulfur sites in polynucleotides. It may also prove to be a valuable reagent for preparing heavy metal derivatives of proteins for x-ray crystallographic study.
四（乙酰氧基汞）甲烷与6-硫代鸟苷的硫原子结合，也与大肠杆菌tRNAVal的4-硫尿苷残基结合。6-硫代鸟苷与四（乙酰氧基汞）甲烷形成4:1的复合物。向四（乙酰氧基汞）甲烷中加入3当量的N,N-二甲基-2-氨基乙硫醇盐酸盐可有效阻断四个汞原子中的三个，使该化合物对6-硫代鸟苷具有单官能性。在适当条件下，无论有无N,N-二甲基-2-氨基乙硫醇盐酸盐，四（乙酰氧基汞）甲烷都能与大肠杆菌tRNAVal中的4-硫尿苷残基结合，而不形成分子间交联。这些结果表明，四（乙酰氧基汞）甲烷将是一种用于标记多核苷酸中硫位点的有用多金属试剂。它也可能被证明是一种用于制备蛋白质重金属衍生物以进行X射线晶体学研究的有价值试剂。
Zotero 插件