Kellner D G, Maves S A, Sligar S G
Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana 61801, USA.
Curr Opin Biotechnol. 1997 Jun;8(3):274-8. doi: 10.1016/s0958-1669(97)80003-1.
Building on the vast knowledge of active site structure and catalytic mechanisms of the P450 monooxygenase systems, significant efforts to utilize the rational design of engineered P450s are emerging as an approach to solve the problems of bioremediation. P450 enzymes are being designed to alter substrate specificities and catalytic efficiency in predefined ways. In addition, random mutagenesis and in vitro evolution are being considered as exciting methods for generating mutant P450s with increased bioremediation abilities.
基于对细胞色素P450单加氧酶系统活性位点结构和催化机制的广泛了解，利用合理设计工程化细胞色素P450的重大努力正在成为解决生物修复问题的一种方法。人们正在设计细胞色素P450酶，以预定义的方式改变底物特异性和催化效率。此外，随机诱变和体外进化被认为是产生具有增强生物修复能力的突变细胞色素P450的令人兴奋的方法。
