Engineering cytochrome P450s for bioremediation.

Building on the vast knowledge of active site structure and catalytic mechanisms of the P450 monooxygenase systems, significant efforts to utilize the rational design of engineered P450s are emerging as an approach to solve the problems of bioremediation. P450 enzymes are being designed to alter substrate specificities and catalytic efficiency in predefined ways. In addition, random mutagenesis and in vitro evolution are being considered as exciting methods for generating mutant P450s with increased bioremediation abilities.