Cai R, Cao X, Ma S
Department of Immunology, Second Military Medical University, Shanghai.
Zhonghua Zhong Liu Za Zhi. 1996 Mar;18(2):97-101.
In the present study, the potential of fibroblast-mediated IL-6 gene therapy for accelerating hematopoietic reconstitution after syngeneic bone marrow transplantation (BMT) in BALB/c mice subjected to total body irradiation at a dose of 7 Gy was investigated. We observed that not only recovery of platelets, WBCs, CFU-GM or CFU-MK in bone marrow in mice treated with IL-6 gene therapy in combination with transplantation of 10(7) syngeneic bone marrow cells was faster than that in mice treated with IL-6 gene therapy in combination with transplantation of 10(6) or 10(5) syngeneic bone marrow cells, but also the number of CFU-S or survival rate in mice treated with IL-6 gene therapy in combination with transplantation of 10(7) syngeneic bone marrow cells was significantly higher. Nevertheless, the number of platelets, WBCs, CFU-GM or CFU-MK in bone marrow, CFU-S and survival rate in mice treated with IL-6 gene therapy in combination with transplantation of syngeneic bone marrow cells were elevated more significantly than that in mice transplanted with syngeneic bone marrow cells alone. These data demonstrated that IL-6 gene therapy could markedly augment hematopoietic reconstitution after syngeneic bone marrow transplantation and the more bone marrow cells transplanted, the better was the effect.
在本研究中，我们探讨了成纤维细胞介导的白细胞介素-6（IL-6）基因治疗对加速受7 Gy全身照射的BALB/c小鼠同基因骨髓移植（BMT）后造血重建的潜力。我们观察到，与移植10⁶或10⁵个同基因骨髓细胞的IL-6基因治疗小鼠相比，移植10⁷个同基因骨髓细胞并联合IL-6基因治疗的小鼠骨髓中血小板、白细胞、粒-巨噬细胞集落形成单位（CFU-GM）或巨核细胞集落形成单位（CFU-MK）的恢复更快，而且移植10⁷个同基因骨髓细胞并联合IL-6基因治疗的小鼠的脾集落形成单位（CFU-S）数量或存活率显著更高。然而，与单纯移植同基因骨髓细胞的小鼠相比，移植同基因骨髓细胞并联合IL-6基因治疗的小鼠骨髓中的血小板、白细胞、CFU-GM或CFU-MK数量、CFU-S及存活率升高更为显著。这些数据表明，IL-6基因治疗可显著增强同基因骨髓移植后的造血重建，且移植的骨髓细胞越多，效果越好。
