Fukae M, Tanabe T, Murakami C, Dohi N, Uchida T, Shimizu M
Department of Biochemistry, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
Adv Dent Res. 1996 Nov;10(2):111-8. doi: 10.1177/08959374960100020201.
The primary structure of the 89-kDa enamelin found in porcine secretory enamel at an early stage of development was investigated. The fragments of the enamelin cDNA were amplified by polymerase chain-reaction from the first-strand enamelin cDNA, and were sequenced. The results indicated that the 89-kDa enamelin consisted of 627 amino acid residues and had a molecular mass of 70,448. A hydrophobic domain is located in the region of the 21st-62nd amino acid residues of the molecule. Acidic domains are located in two regions of the molecule-one in the region of the 135th-238th amino acid residues and the other in the C-terminal region. A basic domain is located in the region of the 239th-360th amino acid residues. The results also indicated that the low-molecular-weight enamelins were fragments derived from a prototype enamelin.
对猪分泌性釉质发育早期发现的89-kDa釉原蛋白的一级结构进行了研究。通过聚合酶链反应从第一链釉原蛋白cDNA中扩增出釉原蛋白cDNA片段，并进行测序。结果表明，89-kDa釉原蛋白由627个氨基酸残基组成，分子量为70448。疏水结构域位于分子第21-62个氨基酸残基区域。酸性结构域位于分子的两个区域，一个在第135-238个氨基酸残基区域，另一个在C末端区域。碱性结构域位于第239-360个氨基酸残基区域。结果还表明，低分子量釉原蛋白是源自原型釉原蛋白的片段。
