Fong C D, Hammarström L, Lundmark C, Wurtz T, Slaby I
Karolinska Institute, Center for Oral Biology, Huddinge, Sweden.
Adv Dent Res. 1996 Nov;10(2):195-200. doi: 10.1177/08959374960100021301.
We have recently identified a novel RNA sequence in ameloblasts, coding for amelin (Cerny et al., 1996). In the present paper, its expression has been compared with that of amelogenin in developing incisors and molars of rats, by means of in situ hybridization of paraffin sections. The RNAs for both amelin and amelogenin were highly expressed in secretory ameloblasts. The expression of RNA for amelogenin gradually decreased in the post-secretory ameloblasts. In contrast, the RNA expression for amelin remained high in post-secretory ameloblasts up to the stage of fusion between dental and oral epithelia at the time of tooth eruption. We suggest that amelin might be involved in the mineralization of enamel or in the attachment of ameloblasts to the enamel surface. The whole-mount in situ hybridization procedure is described for the first time in dental research. It proved to be a useful method and confirmed the results of the conventional in situ hybridization.
我们最近在成釉细胞中鉴定出一种新的RNA序列，它编码amelin（Cerny等人，1996年）。在本文中，通过石蜡切片的原位杂交技术，比较了其与牙釉蛋白在大鼠正在发育的切牙和磨牙中的表达情况。amelin和牙釉蛋白的RNA在分泌期的成釉细胞中均高度表达。牙釉蛋白的RNA表达在分泌后成釉细胞中逐渐降低。相比之下，amelin的RNA表达在分泌后成釉细胞中一直保持较高水平，直到牙齿萌出时牙上皮和口腔上皮融合阶段。我们认为amelin可能参与釉质矿化或成釉细胞与釉质表面的附着过程。全组织原位杂交程序在牙科研究中首次被描述。它被证明是一种有用的方法，并证实了传统原位杂交的结果。
