Grüntzig J, Mehlhorn H, Steiger R
Universitätsaugenklinik Düsseldorf.
Klin Monbl Augenheilkd. 1997 Jan;210(1):58-61. doi: 10.1055/s-2008-1035015.
The accepted fundamental element of the initial lymphatic system is a tubular structure (lymphatic capillary), which is present either in the form of a finger-shaped blind-ending protuberance, or in the nature of a plexiform composite arrangement. We were able to demonstrate experimentally that the finger-shaped protuberances, which are known as "initial segments" are in fact temporary filling states. As the filling process continues, these vascular elements become intermediate segments, via which other parts of the vascular network take up the dye. To date there have been no investigations of the internal structure of these apparently blind-ending vessel segments. In this study, lymphographically represented "terminal segments" should be cut away for the purpose of examining the fornix by scanning electron microscopy. Is the internal surface of the fornix regular and unbroken, or are there fissure-like structures which could explain the observed filling processes?
In conjunctiva of bovine eyes (n = 80), interstitial double-contrast lymphography (Berlinblue solution/air) under a slit-lamp microscope was used specifically to search for finger-shaped terminal segments. The conjunctivae had previously been prepared by fixing in polymeric resins. A proportion (n = 21) of the specimens were amenable to examination under the scanning electron microscope.
Notwithstanding the observed great variation in the shape of the lymphographically obtained blind-ending structures-in the form of terminations shaped variously like fingers, balloons, domes, pistons or pyramids, terminations with two humps, and terminations shaped like spear heads-scanning electron microscopy revealed within the fornices many relatively uniformly shaped structures in the form of fissures, configured with lips and saw-tooth edges, rather like zip fasteners. These findings are suggestive of preformed connections to neighbouring segments. This appears to be another element, in addition to the familiar flap-like structures, for controlling the circulation of lymph. Does the "initial part" of the lymphatic system now have to be redefined?
公认的初始淋巴系统的基本组成部分是管状结构（毛细淋巴管），它以指状盲端突起的形式存在，或以丛状复合排列的形式存在。我们通过实验证明，被称为“初始段”的指状突起实际上是暂时的充盈状态。随着充盈过程的持续，这些血管成分会变成中间段，血管网络的其他部分通过这些中间段摄取染料。迄今为止，尚未对这些明显盲端的血管段的内部结构进行研究。在本研究中，为了通过扫描电子显微镜检查穹窿，应切除淋巴造影显示的“终末段”。穹窿的内表面是规则且完整的，还是存在可以解释观察到的充盈过程的裂隙样结构？
在牛眼结膜（n = 80）中，在裂隙灯显微镜下进行间质双对比淋巴造影（柏林蓝溶液/空气），专门用于寻找指状终末段。结膜此前已用聚合树脂固定。一部分（n = 21）标本适合在扫描电子显微镜下检查。
尽管观察到淋巴造影获得的盲端结构形状差异很大，其末端形状各异，如手指、气球、穹顶、活塞或金字塔形，有两个隆起的末端，以及矛头形末端，但扫描电子显微镜显示，在穹窿内有许多形状相对一致的裂隙样结构，其边缘有唇状和锯齿状，很像拉链。这些发现提示与相邻段存在预先形成的连接。这似乎是除了常见的瓣状结构之外，控制淋巴循环的另一个要素。淋巴系统的“初始部分”现在是否需要重新定义？
