Mazhdrakova I P, Balabanov T B
Augenklinik, Medizinische Universität, Sofia, Bulgarien.
Klin Monbl Augenheilkd. 1997 Nov;211(5):335-7. doi: 10.1055/s-2008-1035143.
The aim of the investigation is a combination of a harmless and easy method of visualisation with a suitable way or recording of the dynamic processes of flow of dye in the lymphatic vessels of the conjunctiva in human eyes in vivo.
A visualisation method of injecting subconjunctivally 0.10 ml of a 2.5% solution of Patentblau V, was employed to study the initial lymphatic vessels (LV) of conjunctiva. The penetration of the dye in the LV was observed through an operating microscope and was filmed with the video camera attached. The anatomy of the initial LV of the conjunctiva, their size, the speed of lymph flow and the time necessary for the diffusion of the dye through the LV walls were recorded.
The method was applied to 16 patients suffering from various ophthalmologic and general disorders. Well-shaped lymph vessels in the conjunctiva are located circularly around the limbus, at a distance of 3-5 mm. These LV often resemble rosaries consisting of separate segments (lymphangions). The lymph flow varies in speed. When the valves shut completely, the speed of the lymph flow in the respective lymphangion equals 0, but when the valves open, the lymph may gradually or rapidly stream into the next lymphangion. The LV vary greatly in size, which depends mainly on the quantity of liquid that has penetrated inside them.
The time necessary for the dye to diffuse through the LV walls also varies greatly and depends on the type and dynamics of the disorder of the anterior eye segment, as well as on the general vascular disorders of individual patients and their effect on the permeability of the LV walls.
本研究旨在将一种无害且简便的可视化方法与一种合适的方式相结合，以记录人眼结膜淋巴管内染料流动的动态过程。
采用结膜下注射0.10 ml 2.5%专利蓝V溶液的可视化方法，研究结膜的初始淋巴管（LV）。通过手术显微镜观察染料在LV中的渗透情况，并用连接的摄像机进行拍摄。记录结膜初始LV的解剖结构、大小、淋巴流动速度以及染料透过LV壁扩散所需的时间。
该方法应用于16例患有各种眼科和全身性疾病的患者。结膜中形态良好的淋巴管呈环状位于角膜缘周围，距离为3 - 5 mm。这些LV通常类似由独立节段（淋巴管腔）组成的念珠。淋巴流动速度各异。当瓣膜完全关闭时，相应淋巴管腔内的淋巴流动速度为0，但当瓣膜打开时，淋巴可逐渐或迅速流入下一个淋巴管腔。LV的大小差异很大，这主要取决于进入其中的液体量。
染料透过LV壁扩散所需的时间也差异很大，这取决于眼前节疾病的类型和动态变化，以及个体患者的全身血管疾病及其对LV壁通透性的影响。
