Ding M, Kinoshita Y, Kishi K, Nakata H, Hassan S, Kawanami C, Sugimoto Y, Katsuyama M, Negishi M, Narumiya S, Ichikawa A, Chiba T
Department of Medicine, Kobe University School of Medicine, Japan.
Prostaglandins. 1997 Mar;53(3):199-216. doi: 10.1016/s0090-6980(97)00015-4.
In order to study the role of prostaglandin in the regulation of the gastrointestinal functions, gene expression of prostaglandin receptors along the rat gastrointestinal tracts were investigated.
Rats were used for the study. The combination of counterflow elutriation separation of mucosal cells and Northern blot analysis was used to detect the gene expression of prostaglandin receptors in gastrointestinal tracts.
In small intestine and colon, prostaglandin E2 EP1 and EP3 receptor mRNAs were mainly localized in the deeper intestinal wall containing muscle layers. EP4 receptor gene expression, on the other hand, was detected in the intestinal mucosal layer. In the stomach, EP1 mRNA was detected in gastric muscle layers, whereas EP3 and EP4 receptor gene expression was mainly present in the gastric mucosal layer containing epithelial cells. In gastric epithelial cells, parietal cells were found to have both EP3 and EP4 receptors. At lower concentrations, prostaglandin E2 inhibited gastric acid secretion by parietal cells probably through EP4 receptors. At higher concentrations, however, it stimulated it. On the other hand, mucous cells possessed only EP4 receptor mRNA.
Thus, it is suggested that prostaglandin E2 modulates gastrointestinal functions through at least three different prostaglandin receptors (EP1, EP3, and EP4), each of which has a distinct contribution in the gastrointestinal tract.
为了研究前列腺素在胃肠功能调节中的作用，对大鼠胃肠道中前列腺素受体的基因表达进行了研究。
使用大鼠进行该研究。采用黏膜细胞逆流淘析分离与Northern印迹分析相结合的方法检测胃肠道中前列腺素受体的基因表达。
在小肠和结肠中，前列腺素E2的EP1和EP3受体mRNA主要定位于包含肌层的肠壁深层。另一方面，在肠黏膜层检测到EP4受体基因表达。在胃中，在胃肌层检测到EP1 mRNA，而EP3和EP4受体基因表达主要存在于包含上皮细胞的胃黏膜层。在胃上皮细胞中，发现壁细胞同时具有EP3和EP4受体。在较低浓度下，前列腺素E2可能通过EP4受体抑制壁细胞的胃酸分泌。然而，在较高浓度下，它会刺激胃酸分泌。另一方面，黏液细胞仅拥有EP4受体mRNA。
因此，提示前列腺素E2通过至少三种不同的前列腺素受体（EP1、EP3和EP4）调节胃肠功能，每种受体在胃肠道中都有独特的作用。
