四环素调控的真核生物转录：具有分级反式激活潜能的新型反式激活因子。
Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential.
作者信息
Baron U, Gossen M, Bujard H
机构信息
ZMBH, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
出版信息
Nucleic Acids Res. 1997 Jul 15;25(14):2723-9. doi: 10.1093/nar/25.14.2723.
Abstract
Several tetracycline-controlled transactivators (tTA) were generated which differ in their activation potential by >3 orders of magnitude. The transactivators are fusions between the Tet repressor and minimal transcriptional activation domains derived from Herpes simplex virus protein 16 (VP16). By reducing the VP16 moiety of the previously described tTA to 12 amino acids, potential targets for interactions with various cellular transcription factors were eliminated, as were potential epitopes which may elicit a cellular immune response. When compared with the originally described tTA, these new transactivators are tolerated at higher intracellular concentrations. This will facilitate establishment of tet regulatory systems under a variety of conditions, but particularly when cell type-restricted tetracycline-controlled gene expression is to be achieved in transgenic organisms via homologous recombination.
摘要
构建了几种四环素调控反式激活因子（tTA），它们的激活潜力相差超过3个数量级。这些反式激活因子是四环素阻遏物与源自单纯疱疹病毒蛋白16（VP16）的最小转录激活结构域的融合体。通过将先前描述的tTA的VP16部分减少到12个氨基酸，消除了与各种细胞转录因子相互作用的潜在靶点，以及可能引发细胞免疫反应的潜在表位。与最初描述的tTA相比，这些新的反式激活因子在更高的细胞内浓度下仍可耐受。这将有助于在各种条件下建立四环素调控系统，特别是当要通过同源重组在转基因生物中实现细胞类型受限的四环素调控基因表达时。
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