Mahloogi H, Behe M J
Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
Biochem Biophys Res Commun. 1997 Jun 27;235(3):663-8. doi: 10.1006/bbrc.1997.6858.
We have measured the ability of oligoadenosine tracts 25 base pairs in length to influence nucleosome formation. Such tracts can cause DNA to bind in nucleosomes at higher temperatures with a free energy up to 1 kcal/mol more favorable than heterogenous-sequence DNA. Furthermore, the position of the oligoadenosine tract affects the free energy of binding, with the most favorable position occurring at the dyad axis.
我们已经测量了长度为25个碱基对的寡腺苷酸序列影响核小体形成的能力。这些序列能够使DNA在更高温度下结合于核小体中，其自由能比异源序列DNA更有利，最高可达1千卡/摩尔。此外，寡腺苷酸序列的位置会影响结合自由能，最有利的位置出现在二分体轴处。
