Schmidt W N, Wu P, Han J Q, Perino M J, LaBrecque D R, Stapleton J T
Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City, USA.
J Infect Dis. 1997 Jul;176(1):20-6. doi: 10.1086/514024.
Previous experiments using a cationic surfactant to detect hepatitis C virus (HCV) RNA in whole blood (WB) suggested that WB was a more plentiful source of viral RNA than was plasma. The relative HCV RNA titers in WB, plasma, peripheral blood mononuclear cells (PBMC), neutrophils, and red blood cells (RBC)/platelets from 10 patients with chronic HCV infection were compared. WB contained significantly more HCV RNA than plasma, which contained more HCV RNA than PBMC, neutrophils, or RBC/platelets (P < .001). To determine if this increased sensitivity was clinically relevant, results of WB and plasma HCV RNA assays were compared with commercial quantitative and qualitative plasma HCV RNA assay results obtained for patients receiving interferon therapy. WB was significantly more sensitive than commercial plasma reverse transcription-polymerase chain reaction for detecting HCV RNA (P < .005). These data indicate that a significant proportion of HCV RNA in peripheral blood is not identified by standard plasma RNA detection methods.
先前使用阳离子表面活性剂检测全血（WB）中丙型肝炎病毒（HCV）RNA的实验表明，与血浆相比，全血是病毒RNA更丰富的来源。比较了10例慢性丙型肝炎病毒感染患者的全血、血浆、外周血单个核细胞（PBMC）、中性粒细胞以及红细胞（RBC）/血小板中的相对HCV RNA滴度。全血中的HCV RNA明显多于血浆，而血浆中的HCV RNA又多于PBMC、中性粒细胞或RBC/血小板（P <.001）。为了确定这种提高的敏感性是否具有临床相关性，将全血和血浆HCV RNA检测结果与接受干扰素治疗患者的商业定量和定性血浆HCV RNA检测结果进行了比较。在检测HCV RNA方面，全血比商业血浆逆转录-聚合酶链反应明显更敏感（P <.005）。这些数据表明，外周血中相当一部分HCV RNA无法通过标准血浆RNA检测方法检测到。
