Evers B, Thiem J
Institut für Organische Chemie, Universität Hamburg, Germany.
Bioorg Med Chem. 1997 May;5(5):857-63. doi: 10.1016/s0968-0896(97)00031-x.
Maltopentaose was immobilized on silica gel and used as a recyclable primer in the reaction of glycogen phosphorylase with D-glucal. An improved method to obtain 2-deoxy-alpha-D-arabino-hexopyranosyl phosphate (4) by using this catalyst as well as the specific synthesis of low molecular weight, water-soluble 2-deoxy-maltooligosaccharides (12, 13, 14 and 15) are described. Further investigations with modified phosphorylase substrates showed that mannosyl phosphate (16) can be slowly transferred to the primer maltotetraose. alpha-1,4-Mannosyl-maltotetraose (17) and its degradation product alpha-1,4-mannosyl-maltose (18) were identified.
麦芽五糖被固定在硅胶上，并用作糖原磷酸化酶与D-葡糖醛反应中的可循环引物。描述了一种使用该催化剂获得2-脱氧-α-D-阿拉伯六吡喃糖基磷酸酯（4）的改进方法，以及低分子量水溶性2-脱氧麦芽寡糖（12、13、14和15）的具体合成方法。对修饰的磷酸化酶底物的进一步研究表明，甘露糖基磷酸酯（16）可以缓慢转移到引物麦芽四糖上。鉴定出了α-1,4-甘露糖基麦芽四糖（17）及其降解产物α-1,4-甘露糖基麦芽糖（18）。
