用磷酸化酶合成2-脱氧-α-D-阿拉伯己吡喃糖基磷酸酯和2-脱氧麦芽寡糖

Synthesis of 2-deoxy-alpha-D-arabino-hexopyranosyl phosphate and 2-deoxy-maltooligosaccharides with phosphorylase.

作者信息

Evers B, Mischnick P, Thiem J

机构信息

Institut für Organische Chemie, Universität Hamburg, Germany.

出版信息

Carbohydr Res. 1994 Sep 15;262(2):335-41. doi: 10.1016/0008-6215(94)84189-6.

DOI:10.1016/0008-6215(94)84189-6

PMID:7982224

Abstract

A convenient one-step synthesis of 2-deoxy-alpha-D-arabino-hexopyranosyl phosphate on a millimolar scale is described by reaction of potato phosphorylase with D-glucal at equimolar phosphate concentration. Furthermore, in the presence of catalytic amounts of phosphate, a 2-deoxy-maltooligosaccharide is obtained from maltotetraose and D-glucal. The water-insoluble oligosaccharide was isolated and characterized by 1H and 13C NMR spectroscopy. An average dp of 20 was thus determined.

摘要

通过马铃薯磷酸化酶与D-葡糖醛在等摩尔磷酸盐浓度下反应，描述了一种方便的毫摩尔规模一步合成2-脱氧-α-D-阿拉伯己吡喃糖基磷酸酯的方法。此外，在催化量的磷酸盐存在下，从麦芽四糖和D-葡糖醛获得了2-脱氧麦芽低聚糖。分离出不溶于水的低聚糖，并通过1H和13C NMR光谱进行表征。由此确定平均聚合度为20。