Zhao G H, Frère J M
Centre d'Ingénierie des Protéines and Laboratoire d'Enzymologie, Institut de Chimie, B6, Université de Liège au Sart Tilman, Belgium.
J Enzyme Inhib. 1997 Feb;11(4):245-64. doi: 10.3109/14756369709027654.
Reversible inhibition phenomena are analyzed for enzymatic systems involving covalent intermediates, where the inhibitor can bind to the pure enzyme, the Henri-Michaelis complex or the covalent intermediate, or to two or three of these enzyme forms. Classical competitive, non-competitive or un-competitive phenomena can be observed in some cases but unexpected features are also observed. Complex phenomena sometimes prevail where increased kcat/Km values can be accompanied by decreased or increased kcat values.
对涉及共价中间体的酶系统的可逆抑制现象进行了分析，其中抑制剂可与纯酶、亨利-米氏复合物或共价中间体结合，或与这些酶形式中的两种或三种结合。在某些情况下可观察到经典的竞争性、非竞争性或反竞争性现象，但也观察到了意外特征。有时会出现复杂现象，即kcat/Km值增加的同时kcat值可能降低或增加。
