Stevenson B, Casjens S, van Vugt R, Porcella S F, Tilly K, Bono J L, Rosa P
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA.
J Bacteriol. 1997 Jul;179(13):4285-91. doi: 10.1128/jb.179.13.4285-4291.1997.
We have mapped the genes encoding the antigenic lipoproteins OspE and OspF to an approximately 18-kb circular plasmid in Borrelia burgdorferi N40. Sequencing and restriction mapping have revealed that this plasmid, cp18, is homologous to an 18-kb region of the cp32 circular plasmids found in the Lyme disease spirochetes. Our data show that cp18 may have arisen from an ancestral cp32 plasmid by deletion of a 14-kb region of DNA, indicating that a significant portion of the cp32 plasmid is not essential in cis for plasmid maintenance. These findings suggest that a relatively small recombinant plasmid capable of being stably maintained in B. burgdorferi could be constructed from a cp32 plasmid.
我们已将编码抗原性脂蛋白OspE和OspF的基因定位到伯氏疏螺旋体N40中的一个约18 kb的环状质粒上。测序和限制性图谱分析表明，该质粒cp18与在莱姆病螺旋体中发现的cp32环状质粒的一个18 kb区域同源。我们的数据表明，cp18可能是由一个祖先cp32质粒通过缺失14 kb的DNA区域而产生的，这表明cp32质粒的很大一部分在顺式条件下对于质粒维持并非必需。这些发现表明，可以从cp32质粒构建出一个能够在伯氏疏螺旋体中稳定维持的相对较小的重组质粒。
